Epitaxial nucleation of protein crystal on poly-L-lysine modified surface

The effects of microscope slides coated with poly-l-lysine (PLL) on the nuc leation of tetragonal hen egg-white lysozyme (E.C. 3.2.1.17. HEWL) have bee n investigated using the batch method. It was found that the PLL-coated gla ss substrate selectively controlled the orientation of tetragonal lysozyme crystals to either {110} or {101} form depending on supersaturation. The to tal number of crystals retained on the coated glass substrate decreased by 20% compared to that of a normal glass substrate.