Determination of rendering plant sterilization conditions using a commercially available ELISA test kit developed for detection of cooked beef

A commercially available enzyme-linked immunosorbent assay (ELISA) test kit developed for detection of cooked beef in meat samples was used to determi ne appropriate heat treatment of rendered materials. An improved extraction procedure increased the absolute difference in R-values between 2 rendered materials treated under different conditions (average temperature 129 and 134 degreesC, respectively). To evaluate the influence of the main steriliz ation parameters on ELISA results, a factorial design approach was used. Th e parameters investigated were temperature, time, particle size, and meat c omposition. Lean meat samples containing beef and pork were sterilized unde r strictly controlled conditions in a laboratory autoclave. The experiments demonstrated that the R-values obtained with the ELISA test kit for beef a re strongly influenced by temperature and time, whereas particle size has a minor influence. The proportion of bovine material did not have any impact on R-values. Autoclave-processed lean meat samples were analyzed by using an ELISA test kit for pork, which was validated in a collaborative trial. T he ELISA test kit for pork proved to be more sensitive for the investigated parameters, thus verifying and extending previous investigations.