T cell lines generated with type II collagen proliferate in an autologous mixed lymphocyte response

Collagen-induced arthritis (CIA) is a T cell-dependent disease induced in s usceptible rodents by immunizing with bovine type II collagen (bCII). In or der to study T cell responses, a programme to generate bCII-specific T cell lines from arthritic rats was initiated. Lymph node cells from bCII-immune WA/KIR/kcl rats were cultured with bCII in vitro, and the T cells were iso lated and restimulated with bCII-pulsed antigen presenting cells (APC) (thy mus cells or splenic low density cells). However, T cells, generated initia lly to bCII, subsequently proliferated upon co-culture with syngeneic APC e ven in the absence of bCII This suggests that exposure to bCII resulted in the activation of a population of self-reactive T cells which proliferate i n an autologous mixed lymphocyte response. In contrast, short-term T cell l ines generated to ovalbumin, heat-denatured bCII and the collagen peptide b CII(184-198) proliferated in response to specific anti-en-pulsed APC withou t demonstrating self-reactivity. Since denatured bCII and bCII(184-198) pep tide are not arthritogenic and failed to generate self reactivity in vitro, this suggests that the native triple helical conformation of bCII is requi red for stimulating autoreactive T cell responses. (C) 2001 Academic Press.