Penicillin acylase purification with the aid of pseudo-affinity chromatography

Authors
Fitton, V Verdoni, N Sanchez, J Santarelli, X
Citation
V. Fitton et al., Penicillin acylase purification with the aid of pseudo-affinity chromatography, J BIOCH BIO, 49(1-3), 2001, pp. 553-560
Citations number
29
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS
ISSN journal
0165022X → ACNP
Volume
49
Issue
1-3
Year of publication
2001
Pages
553 - 560
Database
ISI
SICI code
0165-022X(20011030)49:1-3<553:PAPWTA>2.0.ZU;2-7
Abstract
The introduction of affinity chromatography has opened a new dimension in p rotein purification. This article reviews the current techniques used in th e penicillin acylase purification process, especially pseudo-affinity chrom atography. A profile for a suitable ligand is established. An aromatic ring and the presence of one or several amino groups seem essential for proper interaction. Immobilized metal affinity chromatography now seems to be a go od competitor. (C) 2001 Elsevier Science B.V. All rights reserved.