Affinity purification of DNA-binding proteins

The focus of this review is on DNA affinity chromatography, which is the mo st powerful tool for purification of DNA binding proteins. The use of nonsp ecific-, sequence specific- and single stranded-DNA affinity columns in pur ification of various DNA binding proteins is discussed. The purification st rategies for transcription factors, restriction enzymes, telomerases, DNA a nd RNA polymerase and DNA binding antibodies are described. Different appli cations of DNA affinity chromatography are presented. (C) 2001 Elsevier Sci ence B.V. All rights reserved.