P. Parizek et al., Similar turnover and shedding of the cellular prion protein in primary lymphoid and neuronal cells, J BIOL CHEM, 276(48), 2001, pp. 44627-44632
The cellular prion protein (PrPc) is essential for pathogenesis and transmi
ssion of prion diseases. Although prion replication in the brain is accompa
nied by neurodegeneration, prions multiply efficiently in the lymphoreticul
ar system without any detectable pathology. We have used pulse-chase metabo
lic radiolabeling experiments to investigate the turnover and processing of
PrPc in primary cell cultures derived from lymphoid and nervous tissues. S
imilar kinetics of PrPc degradation were observed in these tissues. This in
dicates that the differences between these two organs with respect to their
capacity to replicate prions is not due to differences in the turnover of
PrPc. Substantial amounts of a soluble form of PrP that lacks the glycolipi
d anchor appeared in the medium of splenocytes and cerebellar granule cells
. Soluble PrP was detected in murine and human serum, suggesting that it mi
ght be of physiological relevance.