D. Kennedy et al., Characterization of G3BPs: Tissue specific expression, chromosomal localisation and rasGAP(120) binding studies, J CELL BIOC, 84(1), 2002, pp. 173-187
The G3BP (ras-GTPase-Activating Protein SH3-Domain-Binding Protein) family
of proteins has been implicated in both signal transduction and RNA-metabol
ism. We have previously identified human G3BP-1, G3BP-2, and mouse G3BP-2.
Here, we report the cloning of mouse G3BP-1, the discovery of two alternati
vely spliced isoforms of mouse, and human G3BP-2 (G3BP-2a and G3BP-2b), and
the chromosomal localisation of human G3BP-1 and G3BP-2, which map to 5q14
.2-5q33.3 and 4q12-4q24 respectively. We mapped the rasGAP(120) interactive
region of the G3BP-2 isoforms and show that both G3BP-2a and G3BP-2b use a
n N-terminal NTF2-like domain for rasGAP(120) binding rather than several a
vailable proline-rich (PxxP) motifs found in members of the G3BPs. Furtherm
ore, we have characterized the protein expression of both G3BP-l and G3BP-2
a/b in adult mouse tissues, and show them to be both tissue and isoform spe
cific. (C) 2001 Wiley-Liss, Inc.