Matrix solid-phase dispersion extraction and high-performance liquid chromatographic determination of residual sulfonamides in chicken

Simultaneous determination of the six sulfonamides (SAs) sulfadiazine, sulf adimidine, sulfamonomethoxine, sulfamethoxazole, sulfadimethoxine and sulfa quinoxaline in chicken using matrix solid-phase dispersion (MSPD) with neut ral aluminium oxide as an MSPD sorbent and high-performance liquid chromato graphy (HPLC) is presented. In the present MSPD, six SAs could be isolated by only one step, elution with a 70% (v/v) aqueous ethanol solution, withou t the sorbent conditioning and the sorbent-tissue matrix washing. For the H PLC determination, a LiChrospher 100 RP-8 and a mixture of 1% acetic acid s olution (pH 3.0, in water)-acetonitrile-N,N-dimethylformamide (78:22:5, v/v /v) as the mobile phase with a photodiode array detector were used. Average recoveries were greater than 87.6% with relative standard deviations betwe en 0.5 and 8.6%. The total time and amount of solvent required for the anal ysis of one sample were <1.5 h and < 12 ml, respectively. (C) 2001 Elsevier Science B.V. All rights reserved.