Capillary zone electrophoresis with electrochemical detection - a simple and effective method to analyze oxygen-consuming and peroxide-forming processes

Numerous biological, chemical and photochemical processes consume molecular oxygen, thereby forming peroxides, hydroperoxides and even hydrogen peroxi de. This paper describes a new system for monitoring quasi simultaneously o xygen uptake and peroxide formation. For this purpose capillary electrophor esis with electrochemical detection was connected to a new automated inject or that periodically injects a sample of the reaction mixture into the capi llary electrophoresis system without any contact with the atmosphere. The d evice was tested with some pharmacologically relevant reactions such as enz ymatic oxidation of glucose catalyzed by glucose oxidase, enzymatic lipid o xidation by Epoxygenase and the photodynamic reaction of the anti-inflammat ory drug ketoprofen. (C) 2001 Elsevier Science B.V. All rights reserved.