Evaluation of Stratus (R) CS stat fluorimetric analyser for measurement ofcardiac markers troponin I (cTnl), creatine kinase MB (CK-MB), and myoglobin

Myoglobin, CK-MB, and Troponin I (cTnl) are cardiac muscle necrosis markers that are useful for detecting acute myocardial infarction (AMI). The Strat us (R) CS (Dade Behring, Inc.) is a discrete fluorimetric immunoassay analy ser designed for the determination of the three cardiac markers from a sing le sample of whole blood or plasma. Overall analytical performances of the Stratus (R) CS provided by Dade Behring were evaluated according to the Fre nch Society of Clinical Biology guidelines. Within-run imprecision (n = 20) for the three parameters at three levels gave values under 5%, whereas CVs for between-run imprecision (n = 20) were under 6%. The sensitivities were 0.03 mug/L for cTnl and 0.4 mug/L for CK-MB. Linearities extended from 0-5 0 mug/L for cTnl, 0-140 mug/L for CK-MB, and 1-900 mug/L for myoglobin. The results, particularly those obtained on whole-blood samples, correlated we ll with those obtained on Stratus (R) II. We did not find any interference with haemolysis, icterus, or lipemia. The system was very easy to use, and fulfills the requirements for the analysis of the three cardiac markers in patients with acute chest pain in emergency situations. J. Clin. Lab. Anal. 15:314-318, 2001. (C) 2001 Wiley-Liss, Inc.