Uptake and intracellular processing of PEG-liposomes and PEG-immunoliposomes by Kupffer cells in vitro

Citation
Ga. Koning et al., Uptake and intracellular processing of PEG-liposomes and PEG-immunoliposomes by Kupffer cells in vitro, J LIPOS RES, 11(2-3), 2001, pp. 195-209
Citations number
54
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF LIPOSOME RESEARCH
ISSN journal
08982104 → ACNP
Volume
11
Issue
2-3
Year of publication
2001
Pages
195 - 209
Database
ISI
SICI code
0898-2104(2001)11:2-3<195:UAIPOP>2.0.ZU;2-Y
Abstract
Specific targeting of drugs to for instance tumors or sites of inflammation may be achieved by means of immunoliposomes carrying site-specific antibod ies on their surface. The presence of these antibodies may adversely affect the circulation kinetics of such liposomes as a result of interactions wit h cells of the mononuclear phagocyte system (MPS), mainly represented by ma crophages in liver and spleen. The additional insertion of poly(ethylene gl ycol) chains on the surface of the immunoliposomes may, however, attenuate this effect. We investigated the influence of surface-coupled rat or rabbit antibodies a nd of PEG on the uptake of liposomes by rat Kupffer cells in culture with H -3-cholesteryloleyl ether as a metabolically stable marker. Additionally, w e assessed the effects of surface-bound IgG and PEG on the intracellular pr ocessing of the liposomes by the Kupffer cells, based on a double-label ass ay using the H-3-cholesteryl ether as an absolute measure for liposome upta ke and the hydrolysis of the degradable marker cholesteryl-C-14-oleate as r elative measure of degradation. Attachment of both rat and rabbit antibodies to PEG-free liposomes caused a several-fold increase in apparent size. The uptake by Kupffer cells, howev er, was 3-4 fold higher for the rat than for the rabbit IgG liposomes. The presence of PEG drastically reduced the difference between these liposome t ypes. Uptake of liposomes without antibodies amounted to only about 10% (no n-PEGylated) or less (PEGylated) of that of the immunoliposomes. In contrast to the marked effects of IgG and PEG on Kupffer cell uptake, th e rate of intracellular processing of the liposomes remained virtually unaf fected by the presence of these substances on the liposomal surface. These observations are discussed with respect to the design of optimally fo rmulated liposomal drug preparations, combining maximal therapeutic efficac y with minimal toxicity.