Immunochemical control of the species origin of porcine crude heparin and detection of ovine and caprine materials

As a consequence of the outbreak of bovine spongiform encephalopathy (BSE), ruminants materials have been generally banned from the production of hepa rin. Immunochemical methods have been recently developed for the control of the raw materials used by manufacturers of materials such as porcine mucos a and for the detection of bovine crude heparins. To certify the porcine or igin of crude porcine heparins and to exclude ovine or caprine materials, n ew ELISAs were developed. Rabbit antisera were produced against species-spe cific antigenic contaminants present in crude heparins or in eluted materia ls (EM) from the chromatographic step of the purification process. When ana lysed by line immunoelectrophoresis, these antisera revealed five to eleven antigenic contaminants in the EMs, the major one being the most anodic and predominant antigen in crude heparins. Using the best antisera, competitiv e indirect ELISAs were optimised. They allowed the detection of porcine, ov ine and caprine crude heparins down to a dilution of 0.6 to 1.5 parts per 1 000, with CVs ranging from 3 to 12%. These ELISAs complete the set of immun ological techniques which can be routinely used by heparin manufacturers to secure their supply chain. (C) 2002 Elsevier Science B.V. All rights reser ved.