Fatiguing inspiratory muscle work causes reflex reduction in resting leg blood flow in humans

1. We recently showed that fatigue of the inspiratory muscles via voluntary efforts caused a time-dependent increase in limb muscle sympathetic nerve activity (MSNA) (St Croix et at. 2000). We now asked whether limb muscle va soconstriction and reduction in limb blood flow also accompany inspiratory muscle fatigue. 2. In six healthy human subjects at rest, we measured leg blood flow ((Q) o ver dot (L)) in the femoral artery with Doppler ultrasound techniques and c alculated limb vascular resistance (LVR) while subjects performed two types of fatiguing inspiratory work to the point of task failure (3-10 min). Sub jects inspired primarily with their diaphragm through a resistor, generatin g (i) 60% maximal inspiratory mouth pressure (P-M) and a prolonged duty cyc le (T-I/T-TOT = 0.7); and (ii) 60% maximal P-M and a T-I/T-TOT of 0.4. The first type of exercise caused prolonged ischaemia of the diaphragm during e ach inspiration. The second type fatigued the diaphragm with briefer period s of ischaemia using a shorter duty cycle and a higher frequency of contrac tion. End-tidal P-CO2 was maintained by increasing the inspired CO2 fractio n (F-I,F-CO2) as needed. Both trials caused a 25-40% reduction in diaphragm force production in response to bilateral phrenic nerve stimulation. 3. (Q) over dot (L) and LVR were unchanged during the first minute of the f atigue trials in most subjects: however, (Q) over dot (L) subsequently decr eased (-30%) and LVR increased (50-60%) relative to control in a time-depen dent manner. This effect was present by 2 min in all subjects. During recov ery the observed chan es dissipated quickly (< 30 s). Mean arterial pressur e (MAP; +4-13 mmHg) and heart rate (+16-20 beats min(-1)) increased during fatiguing diaphragm contractions. 4. When central inspiratory motor output was increased for 2 min without di aphragm fatigue by increasing either inspiratory force output (95% of maxim al inspiratory pressure (MIP)) or inspiratory flow rate (5 x eupnoea), (Q) over dot (L), MAP and LVR were unchanged; although continuing the high forc e output trials for 3 min did cause a relatively small but significant incr ease in LVR and a reduction in (Q) over dot (L). 5. When the breathing pattern of the fatiguing trials was mimicked with no added resistance, LVR was reduced and (Q) over dot (L) increased significan tly; these changes were attributed to the negative feedback effects on MSNA from augmented tidal volume. 6. Voluntary increases in inspiratory effort, in the absence of diaphragm f atigue, had no effecton (Q) over dot (L) and LVR, whereas the two types of diaphragm-fatiguing trials elicited decreases in (Q) over dot (L) and incre ases in LVR. We attribute these changes to a metaboreflex originating in th e diaphragm. Diaphragm and forearm muscle fatigue showed very similar time- dependent effects on LVR and (Q) over dot (L).