Characterization of the 5 '-sequence of the mouse fatty acid amide hydrolase

Fatty acid amide hydrolase (FAAH) is critical for degradation of several im portant fatty acid amides including anandamide, an endocannabinoid, as well as oleamide, a sleep-inducing factor. These compounds play roles in divers e physiological processes ranging from memory and learning to the regulatio n of blood pressure. The mechanisms that regulate FAAH expression have not been characterized. A 5'-region of the mouse FAAH with promoter activity wa s isolated from 1.8 kbp of genomic sequence. Characterization of +1 of tran scription of FAAH by RNA ligase mediated-rapid amplification of cDNA ends s howed that FAAH mRNA is transcribed from multiple transcription start sites lacking a TATA-box element. Functional analysis of the FAAH upstream seque nce fused to a luciferase reporter gene revealed a FAAH-promoter construct with tissue specific activity. A 674-bp FAAH-promoter construct was active in N18TG2 (N18) neuro-blastoma cells and C6 glioma cells, lines that have e ndogenous FAAH activity. The same 674-bp FAAH-promoter construct was not ac tive in C2C12 or L6 myogenic cells, two lines that do not have FAAH activit y. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.