Attempt to differentiate Leishmania (Leishmania) amazonensis L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis using the SSR-PCR technique

Authors
Volpini, AC Passos, VMD Romanha, AJ
Citation
Ac. Volpini et al., Attempt to differentiate Leishmania (Leishmania) amazonensis L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis using the SSR-PCR technique, PARASIT RES, 87(12), 2001, pp. 1056-1059
Citations number
28
Categorie Soggetti
Microbiology
Journal title
PARASITOLOGY RESEARCH
ISSN journal
09320113 → ACNP
Volume
87
Issue
12
Year of publication
2001
Pages
1056 - 1059
Database
ISI
SICI code
0932-0113(200112)87:12<1056:ATDL(A>2.0.ZU;2-3
Abstract
The ability to differentiate reference strains of Leishmania. (Leishmania) amazonensis, L. (L.) chagasi, Leishmania (Viannia) braziliensis and L. (V.) guyanensis was evaluated using the simple sequence repeat polymerase chain reaction (SSR-PCR) technique. This technique differentiates the Leishmania species, generating distinct DNA amplicon profiles. The SSR-PCR profiles w ere similar to but more reproducible than those produced by RAPD. SSR-PCR i s presented as an alternative to other molecular methods for the differenti ation of Leishmania species or strains.