APPLICATION OF SINGLE-MOLECULE DETECTION TO DNA-SEQUENCING

Authors
GOODWIN PM CAI H JETT JH ISHAUGRILEY SL MACHARA NP SEMIN DJ VANORDEN A KELLER RA
Citation
Pm. Goodwin et al., APPLICATION OF SINGLE-MOLECULE DETECTION TO DNA-SEQUENCING, Nucleosides & nucleotides, 16(5-6), 1997, pp. 543-550
Citations number
14
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
5-6
Year of publication
1997
Pages
543 - 550
Database
ISI
SICI code
0732-8311(1997)16:5-6<543:AOSDTD>2.0.ZU;2-P
Abstract
A flow cytometric, single molecule approach to DNA sequencing is descr ibed. A single, fluorescently labeled DNA fragment is suspended in a f low stream. An exonuclease is added to sequentially cleave the end bas e into the flow stream where it is detected and identified by laser-in duced fluorescence.