SINGLE-MOLECULE DETECTION OF COUMARIN-120

Authors
BRAND L EGGELING C SEIDEL CAM
Citation
L. Brand et al., SINGLE-MOLECULE DETECTION OF COUMARIN-120, Nucleosides & nucleotides, 16(5-6), 1997, pp. 551-556
Citations number
21
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
5-6
Year of publication
1997
Pages
551 - 556
Database
ISI
SICI code
0732-8311(1997)16:5-6<551:SDOC>2.0.ZU;2-3
Abstract
Two-photon excitation with a mode-locked titanium: sapphire laser at 7 00 nm and confocal fluorescence microscopy have been used to detect si ngle Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resulting in nucleobase-specif ic fluorescence lifetimes. This suggests applications in current proje cts for ultrasenstive DNA characterization.