IN-VITRO SELECTION, STRUCTURAL CHARACTERISTICS, AND PHOTOCLEAVAGE OF AN ISOALLOXAZINE BINDING RNA APTAMER

Authors
HUBER C BURGSTALLER P FAMULOK M
Citation
C. Huber et al., IN-VITRO SELECTION, STRUCTURAL CHARACTERISTICS, AND PHOTOCLEAVAGE OF AN ISOALLOXAZINE BINDING RNA APTAMER, Nucleosides & nucleotides, 16(5-6), 1997, pp. 717-720
Citations number
28
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
5-6
Year of publication
1997
Pages
717 - 720
Database
ISI
SICI code
0732-8311(1997)16:5-6<717:ISSCAP>2.0.ZU;2-V
Abstract
An RNA motif which binds FMN was isolated by in vitro selection of an RNA library. The secondary structure of this RNA contains an internal loop forming an isoalloxazine binding site through non-canonical base pairing. Irradiation with light in presence of FMN induces photocleava ge at G.U base pairs outside the aptamer binding site.