SOLID-PHASE SYNTHESIS, CLONING AND EXPRESSION OF A SYNTHETIC GENE ENCODING A COLLAGEN-LIKE PEPTIDE

Authors
BRUNINGHOFF A SCHLOSSER S GROGER G ORTIGAO JFR SELIGER H
Citation
A. Bruninghoff et al., SOLID-PHASE SYNTHESIS, CLONING AND EXPRESSION OF A SYNTHETIC GENE ENCODING A COLLAGEN-LIKE PEPTIDE, Nucleosides & nucleotides, 16(5-6), 1997, pp. 875-882
Citations number
13
Categorie Soggetti
Biology
Journal title
Nucleosides & nucleotides → ACNP
ISSN journal
07328311
Volume
16
Issue
5-6
Year of publication
1997
Pages
875 - 882
Database
ISI
SICI code
0732-8311(1997)16:5-6<875:SSCAEO>2.0.ZU;2-P
Abstract
A synthetic gene encoding a collagen-like protein (ACMP, artificial ce ll matrix protein) was designed, constructed and assembled by combined solid phase ligation and PCR-methods. The ACMP-gene (232 bp) was clon ed and expressed in an appropiate E. coli expression system. The expre ssed ACMP (14 kD) was purified by affinity chromatography.