Rm. Raphael et al., Fractional occurrence of defects in membranes and mechanically driven interleaflet phospholipid transport - art. no. 051913, PHYS REV E, 6405(5), 2001, pp. 1913
The picture of biological membranes as uniform, homogeneous bileaflet struc
tures has been revised in recent times due to the growing recognition that
these structures can undergo significant fluctuations both in local curvatu
re and in thickness. In particular, evidence has been obtained that a tempo
rary, localized disordering of the lipid bilayer structure (defects) may se
rve as a principal pathway for movement of lipid molecules from one leaflet
of the membrane to the other. How frequently these defects occur and how l
ong they remain open are important unresolved questions. In this report, we
calculate the rate of molecular transport through a transient defect in th
e membrane and compare this result to measurements of the net transbilayer
flux of lipid molecules measured in an experiment in which the lipid flux i
s driven by differences between the mechanical stress in the two leaflets o
f the membrane bilayer. Based on this comparison, we estimate the frequency
of defect occurrence in the membrane. The occurrence of defects is rare: t
he probability of finding a defect in 1.0 mum(2) of a lecithin membrane is
estimated to be similar to6.0 x 10(-6). Based on this fractional occurrence
of defects, the free energy of defect formation is estimated to be similar
to 1.0 x 10(-19) J. The calculations provide support for a model in which
interleaflet transport in membranes is accelerated by mechanically driven l
ipid flow.