High-performance thin layer chromatographic analysis of anti-inflammatory triterpenoids from Boswellia serrata Roxb.

Authors
Krohn, K Rao, MS Raman, NV Khalilullah, M
Citation
K. Krohn et al., High-performance thin layer chromatographic analysis of anti-inflammatory triterpenoids from Boswellia serrata Roxb., PHYTOCH AN, 12(6), 2001, pp. 374-376
Citations number
9
Categorie Soggetti
Plant Sciences
Journal title
PHYTOCHEMICAL ANALYSIS
ISSN journal
09580344 → ACNP
Volume
12
Issue
6
Year of publication
2001
Pages
374 - 376
Database
ISI
SICI code
0958-0344(200111/12)12:6<374:HTLCAO>2.0.ZU;2-7
Abstract
A rapid and simple high-performance thin layer chromatographic (HPTLC) meth od was developed for the simultaneous quantitative estimation of the biolog ically active triterpenoids beta -boswellic acid, 3-O-acetyl-beta -boswelli c acid, 11-keto-beta -boswellic acid and 3-O-acetyl-11-keto-beta -boswellic acid from the gum resin of Boswellia serrata. The assay combines the isola tion and separation of boswellic acid derivatives on silica gel 60F(254)-HP TLC plates with spot visualisation and scanning at 250 nm. Methanol as foun d to be the most appropriate solvent for the exhaustive extraction of boswe llic acid derivatives. Copyright (C) 2001 John Wiley & Sons, Ltd.