The Notch receptor and its ligands are selectively expressed during hematopoietic development in the mouse

Members of the Notch family of transmembrane receptors are found on primiti ve hematopoietic precursors, and Notch ligand expression has been demonstra ted on the surface of stromal cells, suggesting a role for Notch signaling in mammalian blood cell development. The current report examines the expres sion of Notch receptors and their ligands in murine hematopoietic tissues t o determine: A) which blood cell lineages in the adult are influenced by No tch activity, and B) whether fetal hematopoiesis in the embryo involves the Notch pathway. In the adult mouse, a combination of flow cytometry, immuno histochemistry and Northern analysis was used to examine Notch receptor or ligand expression in bone marrow and spleen. In the embryo, Northern analys is and in situ hybridization were used to characterize Notch receptor and l igand expression in fetal liver on embryonic day 12 (E12) through E17, an a ctive period encompassing both erythropoiesis and granulopoeisis. Flow cyto metry demonstrated the presence of Notch1 and Notch2 receptors on bone marr ow-derived myeloid cells but not on erythroid cells positive for the marker , Ter-119. In situ hybridization of E12 through E17 fetal liver demonstrate d widespread expression of Jagged1 and Delta1 in a pattern similar to but l ess abundant than that of the erythropoietin receptor. Taken together with earlier functional results, the current expression data suggest a role for Notch activity in establishing definitive hematopoiesis in fetal liver, as well as a selective use of Notch signaling in adult erythropoiesis and gran ulopoiesis. Notch receptors in the adult are most likely utilized by early erythroid precursors and intermediate-stage granulocytes, but not by termin ally differentiating cells of either subset.