Gentamicin enzyme-linked immunosorbent assay for microdialysis samples

The authors investigated the use of a commercially available gentamicin enz yme-linked immunosorbent assay (ELISA) for the quantitative analysis of gen tamicin concentrations in microdialysis samples. The assay demonstrated goo d accuracy and precision in the concentration range of 100 to 967 pg/mL. Th e developed quantitative ELISA is a highly sensitive and valid method for m easuring gentamicin concentrations in microdialysis samples. This assay may be a useful alternative to other assay methodologies where analysis may be restricted by sample volume requirements and limited sensitivity.