Aerobic biological treatment of a pharmaceutical wastewater: Effect of temperature on cod removal and bacterial community development

The effect of temperature was studied on the efficiency of soluble COD remo val and bacterial community development during the aerobic biological treat ment of a pharmaceutical wastewater. Using wastewater and bacterial inoculu m obtained from the full-scale facility treating this wastewater, batch lab oratory cultures were operated at 5 degreesC intervals from 30 degreesC to 70 degreesC. Following four culture transfers to allow for bacterial acclim ation, residual soluble COD levels were measured and bacterial community fi ngerprints were obtained by denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments. Solub le COD removal efficiency declined as temperature increased from 30 degrees C (62%) to 60 degreesC (38%). Biological treatment of this wastewater faile d to occur at temperatures higher than 60 degreesC. Gradual shifts in bacte rial community structure were detected as temperature increased, including a concomitant reduction in the number of different bacterial populations. T he impact of temperature on a two-stage biological treatment process was al so compared. Better soluble COD removal was achieved when both reactors wer e operated at 30 degreesC compared to a system where the two stages were co nsecutively operated at 55 degreesC and 30 degreesC. These results indicate that operation of aerobic biological wastewater treatment reactors at elev ated temperatures can have adverse effects on process performance. (C) 2001 Elsevier Science Ltd. All rights reserved.