Pressure stability of lipases and their use in different systems

For the investigation of the solvent impact on the enzymes, lipases from th e different sources (Pseudomonas fluorescences, Rhizpous javanicus, Rhizopu s niveus, Candida rugosa and Porcine pancreas) were used. Stability and act ivity of these lipases in aqueous medium in supercritical CO2 and liquid pr opane at 100 bar and 40 degreesC were studied. On the basis of previous results lipases were used for their application in two different systems. The application of the polysulphone membrane in the continuous stirred tank membrane reactor was studied on the model system o f the hydrolysis of oleyl oleate in propane at high pressure. As a catalyst the Candida rugosa lipase was used. The next utilization of lipases was th e use of on silica aerogel self-immobilized lipase from Porcine pancreas as catalyst for esterification reaction in near-critical propane at 40 degree sC and 100 bar.