Autologous hematopoietic stem cell transplantation in chronic myeloid leukemia with different clinical stages

Seven patients with Philadelphia (Ph) chromosome-positive chronic myeloid l eukemia (CML) were treated with an ICE-based regimen plus G-CSF with the ai m of mobilizing and collecting Ph-negative peripheral stem cells in the set ting of an autologous transplant program. Five patients had CIVIL in the fi rst chronic phase and 2 in the accelerated phase. All patients had been pre viously treated with interferon-a. Median value and ranges for harvested mo nonuclear cells, CD34+ cells and CFU-GM, respectively: 5.65 x 10(8)/kg (2.6 1-11.38); 1.48 x 10(6)/kg (0.216-3.5), and 3.43 x 10(4)/kg (0.243-11.6). FI SH was the only useful method for detection of minimal residual disease on apheresis product showing <5% t(9;22) positive cells in 2 cases and < 10% p ositive cells in 4 other cases. Four of seven autologous grafts have been t ransplanted to date. Busulfan conditioning was used in 1 case and TBI/Cy co nditioning in 3 other cases. All patients are alive and well following tran splantation and are on interferon-alpha therapy. Copyright (C) 2001 S. Karg er AG, Basel.