T. Hyotylainen et al., Determination of biogenic amines in wine by multidimensional liquid chromatography with online derivatisation, ANALYST, 126(12), 2001, pp. 2124-2127
An online multidimensional liquid chromatographic system was developed for
online clean-up, derivatisation and separation of biogenic amines in wines.
The system consists of a cation-exchange precolumn, a derivatisation coil
and an analytical column, and a column-switching valve. The entire system c
an be easily automated. The method proved to be quantitative and sensitive.
Limits of detection were below 0.05 mg l(-1) for all amines and linearity
was preserved over the tested range (0.05-15 mg l(-1)). The method was appl
ied to the analysis of red wines of different origin.