Selective extraction of blood plasma exchangeable copper for isotope studies of dietary copper absorption

Measuring mineral absorption by fecal monitoring is labor-intensive and rel ies on good volunteer compliance. Blood indicators of absorption could be a dvantageous and we have developed a method for selective extraction of rece ntly absorbed (exchangeable) copper based on dialysis of plasma with histid ine and subsequent copper extraction using Chelex resin. The potential for measuring copper absorption by transient enrichment of exchangeable copper with the stable isotope Cu-65 from an ingested tracer, was also investigate d. This method was compared with that of the fecal monitoring technique in a human volunteer, who consumed a 6 mg dose of Cu-65 with inhibitors of cop per absorption. Holmium was used as a non-absorbable rare-earth marker of u nabsorbed tracer excretion, allowing estimation of re-secreted Cu-65 (44 mu g d(-1)), and hence calculation of true tracer absorption, which was only 1 0.8%. Monitoring plasma tracer kinetics showed potential for estimation of copper absorption without the need for fecal copper analysis.