Amperometric detection of cytochrome c by capillary electrophoresis at a sol-gel carbon composite electrode

Capillary electrophoresis (CE) was employed for the determination of cytoch rome c using a wall jet amperometric detector consisting a copper(I) oxide- modified sol-gel carbon composite electrode (CCE), which exhibits a sensiti ve electrocatalytic response for the oxidation of cytochrome c. The optimum conditions of separation and detection are 0.08 M NaOH for the separation solution, 12 kV for separation voltage and +0.60 V versus saturated calomel electrode (SCE) for the detection potential. Calibration was linear over t he concentration range 1-600 muM with the limit of detection of 3.4 muM, ba sed on a signal-to-noise ratio (SIN) of 3. (C) 2001 Elsevier Science B.V Al l rights reserved.