Reversible oligonucleotide immobilisation based on biotinylated polypyrrole film

Authors
Dupont-Filliard, A Roget, A Livache, T Billon, M
Citation
A. Dupont-filliard et al., Reversible oligonucleotide immobilisation based on biotinylated polypyrrole film, ANALYT CHIM, 449(1-2), 2001, pp. 45-50
Citations number
15
Categorie Soggetti
Spectroscopy /Instrumentation/Analytical Sciences
Journal title
ANALYTICA CHIMICA ACTA
ISSN journal
00032670 → ACNP
Volume
449
Issue
1-2
Year of publication
2001
Pages
45 - 50
Database
ISI
SICI code
0003-2670(200112)449:1-2<45:ROIBOB>2.0.ZU;2-S
Abstract
In this paper, we describe the design of a new versatile and reversible DNA sensor. The system is based on biotin grafting-units, covalently linked to a polypyrrole matrix, able to anchor large biomolecules, thanks to biotin/ avidin affinity. These grafting-units can be regenerated after the "denatur ation" of the biotin/avidin link allowing to reuse the matrix for the immob ilisation of an other assembly and then the possibility to generate a new s ensor. (C) 2001 Elsevier Science BN. All rights reserved.