Ir. Ramsay et Pc. Pullammanappallil, Protein degradation during anaerobic wastewater treatment: derivation of stoichiometry, BIODEGRADAT, 12(4), 2001, pp. 247-257
The stoichiometry of reactions that describe protein degradation in anaerob
ic treatment systems were investigated. A methodology was developed to desc
ribe protein degradation to organic acids using a single reaction step. The
reactions for individual amino acid fermentation and their mediating organ
isms were reviewed. The dominant fermentation pathways were selected based
on a number of assumptions. Using the amino acid content of a model protein
, it was then possible to determine stoichiometric coefficients for each ma
jor organic acid product in the overall degradation of the protein. The the
oretical coefficients were then compared to those determined from two exper
imental runs on a continuously-fed, well-mixed, laboratory-scale anaerobic
wastewater treatment system. In general, the coefficients compared well thu
s validating the use of a single reaction step for the overall catabolic re
action of protein degradation to organic acids. Furthermore, even when the
protein concentration in feed or the feed flow rate was doubled, the amino
acid fermentation pathways were found to occur predominantly by only one pa
thway. Although the choice of Stickland reactions over uncoupled degradatio
n provided good comparisons, an electron balance showed that only about 40%
of the amino acids could have proceeded coupled to other amino acid reacti
ons. Uncoupled degradation of the remaining amino acids must have relied on
the uptake of hydrogen produced from these reactions by hydrogen-consuming
methane bacteria.