Thermodynamic yield predictions for biodegradation through oxygenase activation reactions

Activation reactions involve modification of recalcitrant substrates to for ms that are more readily degradable. These reactions require specialized en zymes and cosubstrates, including molecular oxygen and reduced electron car riers. In these reactions, microorganisms invest electrons and cannot captu re energy or carbon for synthesis. The subsequent degradation of the interm ediates formed in activation reactions releases electrons, energy, and carb on that the organisms use for growth. The overall yield is reduced due to t he required activation investments. A mathematical method to predict cell y ields of oxygenase activation reactions is developed using electron and ene rgy balances. Predicted yields are compared with experimental yields for me thane, organic chelating agents, and aromatic hydrocarbons.