Integrin expression and the in vitro invasion of RSV-transformed fibroblasts with multiple drug resistance

Cell adhesion to the extracellular matrix proteins, integrin expression and in vitro invasion were investigated in the HET-SR-2SC-MLN line of RSV-tran sformed Syrian hamster fibroblasts and its derivative line 2SC/20-2, posses sing a cross-resistance to colchicine, vinblastine and farmorubicin. As com pared to parental fibroblasts the drug-resistant cells demonstrated a lower adhesion to denatured collagen but did not differ from them in attachment to native collagen, fibronectin and laminin. Resistant fibroblasts are less active in cell surface expression of the alpha nu beta3 integrin, known to have an affinity to denatured collagen and vitronectin, and exhibit a much lower level of in vitro invasion in matrigel. RGD-containing pentapeptide strongly inhibited invasion of the drug-sensitive cells in matrigel while h ad no effect on invasion of the drug-resistant cells.