Investigation of neuron plasmatic membrane state by the method of dynamic phase mycroscopy

The spectrum of optical path difference (OPD) fluctuations depending on cha nges of leech neuron membrane potential level was investigated with the met hod of Dynamic Phase Microscopy (DPM). Regular OPD changes in interval 1-3 Hz caused by neuron plasmatic membrane oscillations were revealed in quiesc ent state. The frequencies appearance probability decreases during membrane depolarization provoked by increasing of extracellular K+ concentration or K+-channels blocking. It was supposed that the neuron membrane OPD fluctua tions changes during K+-depolarization or under acetylcholine influence are caused the changes of membrane proteins and lipids lateral diffusion veloc ity.