Arsenic trioxide induces apoptosis in human T-cell leukemia virus type 1-and type 2-infected cells by a caspase-3-dependent mechanism involving Bcl-2cleavage
R. Mahieux et al., Arsenic trioxide induces apoptosis in human T-cell leukemia virus type 1-and type 2-infected cells by a caspase-3-dependent mechanism involving Bcl-2cleavage, BLOOD, 98(13), 2001, pp. 3762-3769
Treatment of patients with adult T-cell leukemia-lymphoma (ATLL) using conv
entional chemotherapy has limited benefit because human T-cell leukemia vir
us type 1 (HTLV-1) cells are resistant to most apoptosis-inducing agents. T
he recent report that arsenic trioxide induces apoptosis in HTLV-1-transfor
med cells prompted investigation of the mechanism of action of this drug in
HTLV-1 and HTLV-2 interleukin-2-independent T cells and in HTLV-1-immortal
ized cells or in ex vivo ATLL samples. Fluorescence-activated cell sorter a
nalysis, fluorescence microscopy, and measures of mitochondrial membrane po
tential (Delta Psim) demonstrated that arsenic trioxide alone was sufficien
t to induce programmed cell death in all HTLV-1 and -2 cells tested and in
ATLL patient samples. I kappaB-alpha phosphorylation strongly decreased, an
d NF-kappaB translocation to the nucleus was abrogated. Expression of the a
ntiapoptotic protein Bcl-X-L, whose promoter is NF-kappaB dependent, was do
wn-regulated. The collapse of Delta Psim and the release of cytochrome c to
the cytosol resulted in the activation of caspase-3, as demonstrated by th
e cleavage of PARR A specific caspase-3 Inhibitor (Ac-DEVD-CHO) could rever
se this phenotype. The antiapoptotic factor Bcl-2 was then cleaved, convert
ing ft to a Sax-like death effector. These results demonstrated that arseni
c trioxide Induces apoptosis In HTLV-1- and -2-infected cells through activ
ation of the caspase pathway. (C) 2001 by The American Society of Hematolog
y.