E. Cambau et al., Molecular detection of rifampin and ofloxacin resistance for patients who experience relapse of multibacillary leprosy, CLIN INF D, 34(1), 2002, pp. 39-45
Molecular detection of rifampin resistance (rpoB analysis) in Mycobacterium
leprae was determined for 49 patients who experienced relapse of multibaci
llary leprosy and for 34 untreated patients. Molecular detection of ofloxac
in resistance (gyrA analysis) was determined for the 12 patients who experi
enced relapse and who had received ofloxacin. Results of molecular tests we
re compared with the reference susceptibility test in the mouse footpad. Ov
erall, the efficiency of molecular detection-that is, positive DNA amplific
ation-was 95%, whereas that of the in vivo test was 55% (P < .001). Results
of molecular detection and in vivo test were fully concordant when both we
re available-that is, for 35 rifampin-sensitive cases of leprosy (no rpoB m
utation), 4 ofloxacin-sensitive cases (no gyrA mutation), 11 rifampin-resis
tant cases (rpoB missense mutations), and 1 ofloxacin-resistant case (gyrA
mutation). rpoB and gyrA analysis appears to be an effective method for det
ection of rifampin and ofloxacin resistance in patients with leprosy.