High-throughput screening for biocatalysts

Authors
Wahler, D Reymond, JL
Citation
D. Wahler et Jl. Reymond, High-throughput screening for biocatalysts, CURR OPIN B, 12(6), 2001, pp. 535-544
Citations number
43
Categorie Soggetti
Microbiology
Journal title
CURRENT OPINION IN BIOTECHNOLOGY
ISSN journal
09581669 → ACNP
Volume
12
Issue
6
Year of publication
2001
Pages
535 - 544
Database
ISI
SICI code
0958-1669(200112)12:6<535:HSFB>2.0.ZU;2-4
Abstract
Recent progress in high-throughput enzyme assays includes new examples of f luorogenic and chromogenic substrates, fluorescence resonance energy transf er substrates, and applications of the pH and pM indicator methods. Recent developments of Horeau's pseudo-enantiomer derivatisation method to screen enantioselectivities in high-throughput have also been reported.