D. Dormann et Cj. Weijer, Propagating chemoattractant waves coordinate periodic cell movement in Dictyostelium slugs, DEVELOPMENT, 128(22), 2001, pp. 4535-4543
Migration and behaviour of Dictyostelium slugs results from coordinated mov
ement of its constituent cells. It has been proposed that cell movement is
controlled by propagating waves of cAMP as during aggregation and in the mo
und. We report the existence of optical density waves in slugs; they are in
itiated in the tip and propagate backwards. The waves reflect periodic cell
movement and are mediated by cAMP, as injection of cAMP or cAMP phosphodie
sterase disrupts wave propagation and results in effects on cell movement a
nd, therefore, slug migration. Inhibiting the function of the cAMP receptor
cAR1 blocks wave propagation, showing that the signal is mediated by cAR1.
Wave initiation is strictly dependent on the tip; in decapitated slugs no
new waves are initiated and slug movement stops until a new tip regenerates
. Isolated tips continue to migrate while producing waves. We conclude from
these observations that the tip acts as a pacemaker for cAMP waves that co
ordinate cell movement in slugs.