Enhanced production of paclitaxel by semi-continuous batch process (SCBP) in suspension culture of Taxus chinensis

Authors
Choi, HK Yun, JH Kim, SI Son, JS Kim, HR Kim, JH Choi, HJ Hong, SS
Citation
Hk. Choi et al., Enhanced production of paclitaxel by semi-continuous batch process (SCBP) in suspension culture of Taxus chinensis, ENZYME MICR, 29(10), 2001, pp. 583-586
Citations number
13
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
ENZYME AND MICROBIAL TECHNOLOGY
ISSN journal
01410229 → ACNP
Volume
29
Issue
10
Year of publication
2001
Pages
583 - 586
Database
ISI
SICI code
0141-0229(200112)29:10<583:EPOPBS>2.0.ZU;2-R
Abstract
Semi-continuous batch process (SCBP) was developed for the production of pa clitaxel from suspension culture of Taxus chinensis. Cell ages did not affe ct the specific production of paclitaxel, and it was possible to use the ce lls at day 21 as an inoculum for another cycle of production batch. Using S CBP, paclitaxel productions at day 42 were increased from 46.9 at cycle 1 t o 85.5 mg l(-1) at cycle 4, but decreased at cycle 5 and 6. It was elucidat ed that the enhanced production of paclitaxel by SCBP was mainly due to the pre-treatment of silver nitrate. (C) 2001 Elsevier Science Inc. All rights reserved.