Two-dimensional gel electrophoresis of selenized yeast and autoradiographyof Se-75-containing proteins

Two-dimensional high-resolution gel electrophoresis (2DE) has been applied to the fractionation of Se-75-containing proteins in yeast, grown in Se-75- containing medium, and autoradiography was used for detection of the Se-75- containing proteins. Gel filtration and ultrafiltration were used to check whether the selenium sidechains were stable in the presence of the chemical s used for lysis and 2DE. The mass distribution of the selenium-containing proteins was estimated by use of gel filtration and the results were compar ed with the distribution obtained by 2DE. A 2DE map of selenium-containing proteins in yeast is presented, and compared with a total protein map of ye ast.