Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene

Acyl-CoA synthetases (ACSs) play an essential role in fatty acid metabolism . ACS3 is an arachidonate-preferring enzyme expressed in a wide range of hu man tissues including brain, heart, placenta, prostate, skeletal muscle, te stis and thymus. As an initial step to understanding the transcriptional re gulation of the human ACS3 gene, we analyzed the genomic organization and t ranscription units of the human ACS3 gene. Sequence analysis of genomic clo nes demonstrates that the human ACS3 gene spans at least 80.6 kb and contai ns 17 exons. The human ACS3 gene was mapped between the sequence-tagged sit e markers D2S360 and WI-21901. Sequence inspection of the 5'-flanking regio n revealed potential DNA elements including CCAAT, AP-1, Oct-1, GATAs, SRY, CdxA, Nkx-2.5, c-Myb, HSF2, NF-AT, AP-2, NF-Y, and p300. A minimal promote r region required for the expression of the human ACS3 gene in melanoma G36 1 cells was determined. (C) 2001 Elsevier Science B.V. All rights reserved.