Enhanced IL-18 expression in common skin tumors

Citation
H. Park et al., Enhanced IL-18 expression in common skin tumors, IMMUNOL LET, 79(3), 2001, pp. 215-219
Citations number
17
Categorie Soggetti
Immunology
Journal title
IMMUNOLOGY LETTERS
ISSN journal
01652478 → ACNP
Volume
79
Issue
3
Year of publication
2001
Pages
215 - 219
Database
ISI
SICI code
0165-2478(200112)79:3<215:EIEICS>2.0.ZU;2-B
Abstract
Interleukin-18 (IL-18) has been found to have multiple effects upon various cells involved in inflammatory response. Recently we reported that B16 mur ine melanoma cells are able to produce IL-18, which is involved in the regu lation of intracellular reactive oxygen intermediates (ROI) and Fas-ligand expression, indicating that IL-18 plays key role in the tumor activity of m elanoma. In this study, we investigated the pattern of IL-18 expression in the human system. IL-18 production was tested by enzyme linked immunosorben t assay (ELISA) assay in various tumor cell lines, including Raji (Burkett' s lymphoma), IM-9 (B lymphoblast), Jurkat (acute T cell leukemia), SK-MES-1 (squamous cell carcinoma (SCC) cell line), SK-MEL-2, G-361, DM-4, and DX-3 (melanoma cell lines). ELISA tests showed that IL-18 was highly expressed in malignant skin tumors such as SK-MES-1, SK-MEL-2, G-361, DM-4, and DX-3 cell lines, thus suggesting that IL-18 production may be associated with th e malignancy of skin tumors. Here, we report that enhanced IL-18 expression is positively correlated with malignant skin tumors such as SCC and melano ma, suggesting the importance role of IL-18 in malignancy of skin tumors. T aken together, expression of IL-18 by tumor cells in human skin tissue may provide an important clue to understand the pathogenesis of malignant skin tumors. (C) 2001 Elsevier Science B.V. All rights reserved.