Changes in concentrations of type IV collagen and tissue inhibitor of metalloproteinase-1 in patients with paraquat poisoning

Respiratory failure is one of the major causes of death in patients with pa raquat poisoning. In paraquat-poisoned lungs, abnormal extracellular matrix regulation occurs. The aim of the present study is to determine whether se rum concentrations of type IV collagen and tissue inhibitor of metalloprote inase-1 (TIMP-1) are altered during the course of paraquat poisoning and wh ether haemoperfusion therapy affects these concentrations. Twenty-one patie nts were admitted within 3 h after ingestion of paraquat and all patients r eceived direct haemoperfusion therapy. Five out of 21 patients survived and 16 patients died within 28 days. Plasma paraquat concentrations in non-sur vivors (5740 +/- 380 mug l(-1)) were not significantly different from those in survivors (5920 +/- 280 mug l(-1)) before treatment. Haemoperfusion red uced these concentrations in both non-survivors (120 +/- 7 mug l(-1)) as we ll as survivors (136 +/- 9 mug l(-1)) on day 5. Serum concentrations of typ e IV collagen and TIMP-1 in survivors showed little change between day I (t ype IV collagen, 90.4 +/- 3.6 ng ml(-1); TIMP-1, 172.2 +/- 7.0 ng ml(-1)) a nd day 5 (type IV collagen, 92.6 +/- 4.2 ng ml(-1); TIMP-1, 174.2 +/- 7.2 n g ml(-1)). In contrast, these concentrations in non-survivors on day 5 (typ e IV collagen, 143.6 +/- 7.8 mg ml(-1); TIMP-1, 246.8 +/- 13.6 ng ml(-1)) w ere significantly higher than those on day 1 (type IV collagen, 88.4 +/- 4. 2 ng ml(-1), P < 0.01; TIMP-1, 170.6 +/- 9.2 ng ml(-1), P < 0.05). These da ta suggest that serum concentrations of type IV collagen and TIMP-1 may be useful indicators for the development of respiratory failure in patients wi th paraquat poisoning. Copyright (C) 2001 John Wiley & Sons, Ltd.