RecA protein filaments disassemble in the 5 ' to 3 ' direction on single-stranded DNA

Authors
Bork, JM Cox, MM Inman, RB
Citation
Jm. Bork et al., RecA protein filaments disassemble in the 5 ' to 3 ' direction on single-stranded DNA, J BIOL CHEM, 276(49), 2001, pp. 45740-45743
Citations number
40
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN journal
00219258 → ACNP
Volume
276
Issue
49
Year of publication
2001
Pages
45740 - 45743
Database
ISI
SICI code
0021-9258(200112)276:49<45740:RPFDIT>2.0.ZU;2-D
Abstract
RecA protein forms filaments on both single- and double-stranded DNA. Sever al studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-depende nt fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling th e 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrat e unambiguously that RecA filaments disassemble uniquely in the 5' to 3' di rection.