Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay

Authors
Wannet, WJB Reessink, M Brunings, HA Maas, HME
Citation
Wjb. Wannet et al., Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay, J CLIN MICR, 39(12), 2001, pp. 4483-4486
Citations number
31
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
12
Year of publication
2001
Pages
4483 - 4486
Database
ISI
SICI code
0095-1137(200112)39:12<4483:DOPYEB>2.0.ZU;2-D
Abstract
A duplex PCR assay targeting the ail and 16S rRNA genes of Yersinia enteroc olitica was developed to specifically identify pathogenic Y. enterocolitica from pure culture. Validation of the assay was performed with 215 clinical Yersinia strains and 40 strains of other bacterial species. Within an assa y time of 4 h, this assay offers a very specific, reliable, and inexpensive alternative to the conventional phenotypic assays used in clinical laborat ories to identify pathogenic Y. enterocolitica.