Automation of fluorescence-based PCR for confirmation of meningococcal disease

Authors
Diggle, MA Edwards, GFS Clarke, SC
Citation
Ma. Diggle et al., Automation of fluorescence-based PCR for confirmation of meningococcal disease, J CLIN MICR, 39(12), 2001, pp. 4518-4519
Citations number
10
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
12
Year of publication
2001
Pages
4518 - 4519
Database
ISI
SICI code
0095-1137(200112)39:12<4518:AOFPFC>2.0.ZU;2-R
Abstract
A fluoresence-based PCR method was developed, fully automated, and used to confirm infection with Neisseria meningitidis by detection of the meningoco ccus-specific ctrA gene. The method provided a highly sensitive, high-throu ghput assay that was reproducible and less labor-intensive than manual meth ods.