Differentiation of Ehrlichia platys and E-equi infections in dogs by using16S ribosomal DNA-based PCR

Authors
Hancock, SI Breitschwerdt, EB Pitulle, C
Citation
Si. Hancock et al., Differentiation of Ehrlichia platys and E-equi infections in dogs by using16S ribosomal DNA-based PCR, J CLIN MICR, 39(12), 2001, pp. 4577-4578
Citations number
5
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
39
Issue
12
Year of publication
2001
Pages
4577 - 4578
Database
ISI
SICI code
0095-1137(200112)39:12<4577:DOEPAE>2.0.ZU;2-7
Abstract
We have encountered a previously unrecognized specificity problem when usin g the small-subunit ribosomal DNA (16S rDNA)-based PCR primers recommended for use in the identification of Ehrlichia equi in clinical samples. These PCR primers annealed to E. platys 16S rDNA in blood samples containing high levels of E. platys organisms. Therefore, we designed and tested new PCR p rimers for the identification of E. equi.