This in vitro study compared the accuracy of file length measurements made
on calibrated and uncalibrated direct digital images. Endodontic files of k
nown lengths and ISO sizes were used in 10 single-rooted, relatively straig
ht teeth within cadaver specimens. The crowns of the teeth were ground flat
and an orthodontic wire of known length was secured to the coronal surface
. This wire was placed mesiodistally and perpendicular to the root and serv
ed as the reference point for the file measurement and as a calibration ref
erence length. A #20 file was hand-measured to a length that reached the ap
ical third of each tooth. It was inserted and a radiographic image was secu
red. The instrument was remeasured three additional times at different leng
ths on the same tooth and reinserted before each image acquisition. Thus 40
digital images were acquired using a GE X-ray unit and a Schick Computed D
ental Radiography (CDR) #2 sensor. These images were placed in random order
, and an independent, blinded investigator determined the file lengths usin
g on-screen calibrated and uncalibrated measurement of the CDR image with a
straight-line and multiple-line measuring technique. The experimental meas
urements were compared with each other and with the known clinical measurem
ents. A two-way analysis of variance indicated that there was a statistical
ly significant difference showing that the calibrated measurements were mor
e accurate than the uncalibrated measurements (p = 0.0001), and there was n
o significant difference between the straight-line and multiple-line measur
ing techniques (p = 0.14).