Inhibition of vancomycin-resistant enterococci by an in vitro continuous-flow competitive exclusion culture containing human stool flora

Citation
Cj. Donskey et al., Inhibition of vancomycin-resistant enterococci by an in vitro continuous-flow competitive exclusion culture containing human stool flora, J INFEC DIS, 184(12), 2001, pp. 1624-1627
Citations number
15
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Immunology
Journal title
JOURNAL OF INFECTIOUS DISEASES
ISSN journal
00221899 → ACNP
Volume
184
Issue
12
Year of publication
2001
Pages
1624 - 1627
Database
ISI
SICI code
0022-1899(200112)184:12<1624:IOVEBA>2.0.ZU;2-L
Abstract
An in vitro anaerobic continuous-flow competitive exclusion (CFCE) culture model was used to study the ability of human stool flora to inhibit the gro wth of vancomycin-resistant (VR) enterococci (VRE). The CFCE culture was es tablished from a stool sample obtained from a healthy adult. When 10(3) or 10(6) cfu/mL of VR Enterococcus faecium were added to the CFCE culture, the VRE were eliminated within 6 or 9 days, respectively. When 10(7) cfu/mL of the CFCE culture was added to a continuous-flow culture that contained 10( 7) cfu/mL of VRE, the density of VRE was reduced but not eliminated. These data support the hypothesis that the indigenous intestinal flora inhibit gr owth of VRE and suggest that CFCE cultures may be a useful means to study i nteractions between the indigenous flora and VRE.