Diagnosis of Oropouche virus infection by RT-nested-PCR

Authors
Moreli, ML Aquino, VH Cruz, ACR Figueiredo, LTM
Citation
Ml. Moreli et al., Diagnosis of Oropouche virus infection by RT-nested-PCR, J MED VIROL, 66(1), 2002, pp. 139-142
Citations number
16
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF MEDICAL VIROLOGY
ISSN journal
01466615 → ACNP
Volume
66
Issue
1
Year of publication
2002
Pages
139 - 142
Database
ISI
SICI code
0146-6615(200201)66:1<139:DOOVIB>2.0.ZU;2-X
Abstract
Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients, These results show that this RT-nested-PCR is a useful tool for rapid diagn osis of Oropouche fever infections. (C) 2002 Wiley-Liss, Inc.